FlowJo
If you know flow, then you know FlowJo software. Or do you? Strengthen your foundational knowledge and supercharge your skills by mastering plugins.
Registration
The workshop will take place in the Holman Reading Room in the Biomedical library, and is free to UPenn graduate students, postdocs, faculty, and staff.
Overview
FlowJo is an integrated environment for viewing and analyzing flow cytometric data. In addition to gating and statistics, FlowJo has specialized analysis platforms for assays involving DNA/Cell Cycle, Kinetics, and Proliferation, among others. FlowJo’s sophisticated tools allow for generation of statistics, graphs, tables, web pages, and even movies. FlowJo integrates remote data hosting services (LIMS) and supports “cloud” deployment for excellent scaling of performance in a demanding.
Getting set-up
To start the workshop, there are a few things that you’ll need to do. We’ll set aside some time at the start to help guide you through these steps, but you can also do them in advance.
- Make sure you have accepted my invitation for the BD Research Cloud.
- If you’re able, download and install the plugin play bundle
- Get a little wild and download x-shift for clustering and PeacoQC for QC
Agenda
The morning will focus on basic/introductory topics in FlowJo, and the afternoon will delve into more advanced functionality via plugins.
| start | end | topic |
|---|---|---|
| 9:00 | 9:30 | breakfast and set-up |
| 9:30 | 11:30 | Introduction + License support issues + Refresher / introductory training |
| 11:30 | 12:15 | Short discussion on “What is a plugin?” + Hands-on help with installing plugins |
| 12:15 | 1:00 | Lunch break |
| 1:00 | 1:30 | Special considerations for spectral data |
| 1:30 | 3:30 | Hands-on exercise interspersed with slides and discussion |
| 3:30 | 4:00 | Intro to using CellView Lens plugin to incorporate image data from the S8 |
About your instructor
This workshop will be taught by John Quinn, PhD, Director, Science and Product Development at Becton Dickinson (BD) Bioinformatics.
